Have you tried this (CS5):
How do I choose the right sequence settings?
Red, yellow, and green render bars and what they mean
Thanks - Dragging and Dropping works GREAT, however I want to know what I am doing wrong...
I select the correct format, frame rate, audio etc... but a red bar every time...
After dragging & dropping do you still get a red bar?
Well, it seems I have found the problem.
I am shooting in PH 1080/25P, but PP CS5 seems to think the footage is 1080i ??
See the setting of my clip when I create a sequence "from clip"
My camera is set to PH 1080/25P, yet PP seems to think it is 1080i ???
The AF100 is well known as having TRUE 1080/25P or 1080/30P and now 1080/50P (Australia) and 1080/60P (Rest of the world), however PP is showing that it is i, not P?
No - dragging and dropping was FANTSTIC - See my reply about 1080P vs 1080i
There's something in your sequence that isn't the same as the source footage. You'd have to examine both very carefully to see what isn't matching up; it's hard for us to tell from here. The best way to go about it is to create a new sequence from footage, as Joe has already explained.
Inspect your media files to affirm their frame rates.
If Premiere is incorrectly interpreting your media files,
you can right-click the media file in the project panel and
use 'Interpret Footage' to modify the frame rate.
Note: this must be done before adding the clip to a sequence.