I am quantifying stained cells in colonic tissue and I want to know how to set up an action that will select pixels of a certain color so I can get the percentage of those pixels. I will need to be cutting out part of the photo (the non-colonic tissue), so I cannot use the total number of pixels for the percentage.
So far, I know how to make a second layer to use for the pixels I want. I also have instructions for how to cut out the non-colonic tissue. However, I want to make an Action that I can save for later use.
One can record Select > Color Range.
Of course the colors in the files you later process would need to be pretty similar to the original you use to record the Action.