My animation has audio files and there are 7 scenes, between scenes 2 and 3, the audio starts early when I "test movie", but not when I "test scene".
As you could tell, this would throw off the rest of my audio. The error message that pops up says "SWF contains multiple copies of a sound item".
Are you playing the sound in the timeline? Or are you playing the sound using Actionscript? Sound can be attached to the timeline at a keyframe. Look to see if you have attached a sound that you didn't intend in scene 2.