You mean signing certificate on your Smart Card, right? If the certificate on your Smart Card is designed to sign then the answer is "Yes, you can". Certificate Authority that issued a certificates places certain fields in it that may restrict its usage, say, to only encryption, or only signing or only server authentication, etc. The certificate on your Smart Card should not have any usage restrictions incompatible with signing for you to be able to sign with it. You can just try to plug in your Smart Card and look whether Acrobat accepts this certificate for signing.