First, you need to have a duplexing printer, and then you need to set two-sided printing in the printer driver (click the setup button in the Print Booklet dialog to access it). Many users find it easier to export a normal PDF, then use Acrobat's booklet printing.
If nothing else, you can accomplish this by telling the file to print only the ODD or EVEN pages. Then, physically, turn the paper, oriented to what would eventually become the other side of the two.
If this approach is used, you need to understand the printer's "personality." I strongly suggest that you print out and, then, reorient the paper to the other side, to see what it looks like, and figure out what it would be, by using just 2-3 pieces of paper.
Doing this can save paper as well as ink$$$!
You cannot do this with Print Booklet, nor I think with Acrobat's booklet printing. You must have an already imposed file if there is going to be more than one page on each side of the sheet.
You can use one of the several InDesign plug-ins or scripts that are capable of creating an imposed InDesign file or imposed PDF file; then print the result as a normal two-sided print job, using the checkbox in the printer driver specific dialog.