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How are you trying to playback your audio?
After placing it in your timeline, you need to either generate a RAM preview (hit the "zero" key on the numeric keypad), or invoke an "audio only" preview by hitting the "period" key.
Are you using either of those methods?
Are you having trouble with previewing audio or with audio not being included when you render and export a composition?
If the former, read the "Preview video and audio" section of After Effects Help and report back here with detailed results.
If the latter, please tell us more about what format you're exporting to and what your render settings and output module settings are.
As the others already said - AE requires generating previews for audio to be audible properly. During scrubbing in the timeline, you can however hold down the Cmd key to get a choppy and rough idea about sound. If that doesn't solve your problem, presumably you may need to play with the order and priorities of your audio devices on the system level, i.e. disable the internal audio hardware entirely, so AE only sees your extended setup and is forced to use it.