Can anyone point me in the right direction?
Thanks for your help.
1) Bitmaps are not a suitable file type for the web. You will need to open image in your favorite graphics editor, resize image to required height & width for web page, then Save for Web as an optimized GIF, JPG or PNG. The smaller the filesize, the faster your page will load.
2) In DW, Insert > Image. On the Properties Panel you should see the HOTSPOT tools: Selection arrow, rectangle, elipse, polygon.
3) Click the desired tool and drag your mouse around the area you wish to make an active link. I recommend zooming in on the page to make this easier to see.
When you have linked all your hotspot regions on the image map, switch to Code View and add Title attributes (shown in red) to each area shape.
<area shape="rect" coords="337,78,489,193" href="some-link.html" alt="#" title="Tooltip Text goes here"/>
For more info on Image Maps and Hotspots, hit F1 (DW Help).