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Yes. You can either copy entire instances of the effect or you can just copy and paste certain strokes.
Tip: Pressing PP reveals Roto Brush strokes in the Timeline panel.
"You can copy Roto Brush Path properties, and paste them into masks, shapes, and instances of the Paint effect, just like you can with other kinds of paths. If you copy an individual stroke, the Roto Brush span information is not copied; however, if you copy the entire Roto Brush effect instance, it includes the Roto Brush span (and base frame) information."
Will I be able to move the effect as a whole to fit the new shot, say if the second shot is more off center then the first shot?
It's a little bit of a pain, but you can move all of the Roto Brush strokes. I just tried it. I made two copies of a movie, with one offset 50 pixels to the left from the other. I did my Roto Brush work on one and then copied and pasted the entire instance of the Roto Brush effect onto the other. Of course, at this point the results on the offset layer were lousy. But I used the search field in the Timeline panel to filter for 'position', which exposed all of the Position properties for the individual Roto Brush strokes. I Ctrl-clicked them to select all of the strokes' Position properties, and then I dragged the x component of one of them to set it to 50 pixels less. All of the strokes moved left 50 pixels, and the result was good.
Depending on what you're doing, you might have better luck freezing the Roto Brush matte on a duplicate of the original layer and using that as a track matte, and then just moving the whole matte layer.
If you post screenshots, we can give you a better idea beyond my generalizations so far.
Thanks for the advice! Unfortunately After Effects keeps crashing when I try to copy over the rotobrush effect. I really appreciate your help though!